Development of 68 Ga-glycopeptide as an imaging probe for tumor angiogenesis
Journal
Journal of Biomedicine and Biotechnology
Journal Volume
2011
Date Issued
2011
Author(s)
Tsao N.
Wang C.-H.
Her L.-J.
KAI-YUAN TZEN
Chen J.-Y.
Yu D.-F.
Yang D.J.
Abstract
Objective. This study was aimed to study tissue distribution and tumor imaging potential of -6-8Ga-glycopeptide (GP) in tumor-bearing rodents by PET. Methods. GP was synthesized by conjugating glutamate peptide and chitosan. GP was labeled with -6-8Ga chloride for in vitro and in vivo studies. Computer outlined region of interest (counts per pixel) of the tumor and muscle (at the symmetric site) was used to determine tumor-to-muscle count density ratios. To ascertain the feasibility of -6-8Ga-GP in tumor imaging in large animals, PET/CT imaging of -6-8Ga-GP and -1-8F-FDG were conducted in New Zealand white rabbits bearing VX2 tumors. Standard uptake value of tumors were determined by PET up to 45min. To determine blood clearance and half-life of -6-8Ga-GP, blood samples were collected from 10 seconds to 20min. Results. Radiochemical purity of -6-8Ga-GP determined by instant thin-layer chromatography was >95%. Tumor uptake values (SUV) for -6-8Ga-GP and -1-8F-FDG in New Zealand white rabbits bearing VX2 tumors were 3.25 versus 7.04. PET images in tumor-bearing rats and rabbits confirmed that -6-8Ga-GP could assess tumor uptake. From blood clearance curve, the half-life of -6-8Ga-GP was 1.84hr. Conclusion Our data indicate that it is feasible to use -6-8Ga-GP to assess tumor angiogenesis. Copyright 2011 Ning Tsao et al.
SDGs
Other Subjects
chitosan; fluorodeoxyglucose f 18; glutamic acid; glycopeptide ga 68; radiopharmaceutical agent; unclassified drug; diagnostic agent; gallium; glycopeptide; animal experiment; animal model; animal tissue; article; blood analysis; breast cancer; cancer diagnosis; carcinogenesis; computer assisted tomography; controlled study; diagnostic imaging; drug blood level; drug clearance; drug distribution; drug half life; drug purity; drug synthesis; drug uptake; female; in vitro study; isotope labeling; nonhuman; positron emission tomography; rat; thin layer chromatography; animal; blood; breast tumor; capillary electrophoresis; chemistry; computer assisted tomography; Fischer 344 rat; gel chromatography; half life time; neoplasm; neovascularization (pathology); positron emission tomography; rabbit; radiation response; radiography; scintiscanning; tissue distribution; vascularization; Animalia; Oryctolagus cuniculus; Rattus; Rodentia; Animals; Breast Neoplasms; Chromatography, Gel; Dose-Response Relationship, Radiation; Electrophoresis, Capillary; Female; Gallium Radioisotopes; Glycopeptides; Half-Life; Neoplasms; Neovascularization, Pathologic; Positron-Emission Tomography; Rabbits; Rats; Rats, Inbred F344; Tissue Distribution; Tomography, X-Ray Computed
Type
journal article