Enhancement of Nutritional Quality and Anticancer Properties of Cruciferous Sprouts by Supplementation of Chitosan during Cultivation
Date Issued
2013
Date
2013
Author(s)
GOH, CHIOW-PEI
Abstract
Cruciferous sprouts are recognized as health-promoting and cancer preventive foods due to its high content of bioactive phytochemicals. These phytochemicals can be induced in response to biotic stress, such as pathogen invasion. Chitosan, one of the polysaccharides found in the cell wall of specific fungi, exhibits elicitor activity and triggers the biosynthesis of active metabolites. Although the majority of research has been done on native chitosan, but the recetylation of chitosan may have beneficial effects on elicitor property of chitosan. Therefore, the prime objective of this study was to enhance the bioactive compounds and consequently the anticancer properties of cruciferous sprouts by supplementation of chitosan with different degree of acetylation (DA) during cultivation. For this propose, the cruciferous sprouts, including broccoli (Brassica oleracea) and radish (Raphanus sativus), were cultivated with and without supplementation of chitosan. Chitosan with DA11%, DA25% and DA40%, which were prepared by chemical reacetylation, were used as chitosan elicitor and their elicitor activities were compared.
During our study, tolerance test was performed for different concentration of chitosan up to 1% (w/v) for both broccoli and radish sprouts. The chitosan treatment caused a decrease in total fresh weight and dry weight for both sprouts. Further, both the cruciferous sprouts showed different levels of glucosinolate and phenolic content when supplemented with different DA of chitosan. Among the chitosan elicitor, the chitosan with DA 40% showed the highest elicitor activity on broccoli sprouts, which triggered the highest amount of glucosinolate and phenolic compound of sprouts, and also increased the DPPH scavenging activity of sprouts. While, in case of radish sprouts, chitosan with DA11% was the most effective elicitor as compare to others. Further, the chitosan with DA40% and DA11% also significantly enhanced anticancer properties of broccoli and radish sprouts against colon cancer cell lines HT-29. Broccoli showed a 69.95% increase of antiproliferative activity, while radish sprouts showed 30.80% increase. On the other hand, although the chitosan treatment decreased the sprouts yield, the total glucosinolate content in broccoli sprouts and radish sprouts showed an increase of 34.40% and 37.20% by the supplementation of chitosan with DA 40% and chitosan with DA11%, respectively. In conclusion, the best eliciting effect for broccoli sprout is 0.25% of chitosan with DA 40%, and for radish is 0.25% chitosan with DA11%. Thus, fortification of crucifers with chitosan is beneficial in improving the nutritional quality and anticancer properties of broccoli and radish sprouts.
Subjects
十字花科芽菜
抗癌活性
硫配醣體
多酚類物質
抗氧化活性
SDGs
Type
thesis
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