A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome
Journal
Science
Journal Volume
345
Journal Issue
6194
Date Issued
2014-01-01
Author(s)
Lukaszewski, Adam J.
Alberti, Adriana
Sharpe, Andrew
Kilian, Andrzej
Stanca, Antonio Michele
Keller, Beat
Clavijo, Bernardo J.
Friebe, Bernd
Gill, Bikram
Wulff, Brande
Chapman, Brett
Steuernagel, Burkhard
Feuillet, Catherine
Viseux, Claire
Pozniak, Curtis
Rokhsar, Daniel S.
Klassen, Darrin
Edwards, David
Akhunov, Eduard
Paux, Etienne
Alfama, Françoise
Choulet, Frédéric
Kobayashi, Fuminori
Muehlbauer, Gary J.
Quesneville, Hadi
Šimková, Hana
Rimbert, Hélène
Gundlach, Heidrun
Budak, Hikmet
Sakai, Hiroaki
Handa, Hirokazu
Kanamori, Hiroyuki
Batley, Jacqueline
Vrána, Jan
Rogers, Jane
Číhalíková, Jarmila
Doležel, Jaroslav
Chapman, Jarrod
Poland, Jesse A.
Wu, Jianzhong
Khurana, Jitendra
Wright, Jonathan
Bader, Kai Christian
Eversole, Kellye
Barry, Kerrie
McLay, Kirsten
Mayer, Klaus F.X.
Singh, Kuldeep
Clissold, Leah
Pingault, Lise
Couderc, Loïc
Cattivelli, Luigi
Spannagl, Manuel
Kubaláková, Marie
Caccamo, Mario
Mascher, Martin
Bellgard, Matthew
Pfeifer, Matthias
Zytnicki, Matthias
Febrer, Melanie
Alaux, Michael
Martis, Mihaela M.
Loaec, Mikaël
Colaiacovo, Moreno
Singh, Nagendra K.
Glover, Natasha
Guilhot, Nicolas
Stein, Nils
Olsen, Odd Arne
Maclachlan, P. Ron
Chhuneja, Parveen
Wincker, Patrick
Sourdille, Pierre
Faccioli, Primetta
Ramirez-Gonzalez, Ricardo H.
Waugh, Robbie
Šperková, Romana
Knox, Ron
Appels, Rudi
Sharma, Sapna
Ayling, Sarah
Praud, Sebastien
Wang, Shichen
Lien, Sigbjørn
Sandve, Simen Rød
Matsumoto, Takashi
Endo, Takashi R.
Nussbaumer, Thomas
Wicker, Thomas
Tanaka, Tsuyoshi
Scholz, Uwe
Barbe, Valérie
Jamilloux, Véronique
Ogihara, Yasunari
Dubská, Zdeňka
Abstract
An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum) genome has been produced by sequencing isolated chromosome arms. We have annotated 124,201 gene loci distributed nearly evenly across the homeologous chromosomes and subgenomes. Comparative gene analysis of wheat subgenomes and extant diploid and tetraploid wheat relatives showed that high sequence similarity and structural conservation are retained, with limited gene loss, after polyploidization. However, across the genomes there was evidence of dynamic gene gain, loss, and duplication since the divergence of the wheat lineages. A high degree of transcriptional autonomy and no global dominance was found for the subgenomes. These insights into the genome biology of a polyploid crop provide a springboard for faster gene isolation, rapid genetic marker development, and precise breeding to meet the needs of increasing food demand worldwide.
Type
journal article