Substituent Effects on the Photoluminescence Properties of Green Fluorescence Protein-Like Chromophores
Date Issued
2015
Date
2015
Author(s)
Ou, Chun-Lin
Abstract
Meta-amino-substituted green fluorescence protein chromophore (GFPc) (m-DMABDIs) are emissive in aprotic solvents. However hydrogen bonding interactions between the chromophores and protic solvents could induce a fast non-radiative decay process and quench the fluorescence. This thesis is aimed to investigate the fluorescence behave of m-DMABDIs. The E-Z isomerization in the S1 state competes with the fluorescence of GFPc. The meta-amino group could largely increase the barrier for the isomerization and thus enhance the fluorescence. We designed a cyano substituted m-DMABDI to further increase the torsional barrier and to enhance the fluorescence. Another part of this work is to study the intramolecular H-bonded systems, the OH series, by investigating the variable temperature electronic absorption and emission spectra. As the chromophores are sensitive to H-bonding condition, we choose amyloid protein, which plays an important role in Alzheimer''s disease, to investigate if the m-DMABDIs could turn on the fluorescence in response to amyloid protein
Subjects
GFP
green fluorescence protein-like chromophores
substituent effect
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-104-R02223210-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):893d4abd1fa8c72c5401b0b6de339ab4