ASSOCIATION OF HEPATITIS VIRUS INFECTION, ALCOHOL CONSUMPTION AND PLASMA VITAMIN A LEVELS WITH URINARY 8-HYDROXYDEOXYGUANOSINE IN CHEMICAL WORKERS
Resource
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS v.535 n.2 pp.181-186
Journal
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
Journal Volume
v.535
Journal Issue
n.2
Pages
181-186
Date Issued
2003
Date
2003
Author(s)
WONG, RUEY-HONG
YEH, CHING-YING
HSUEH, YU-MEI
WANG, JUNG-DER
LEI, YU-CHEN
CHENG, TSUN-JEN
Abstract
Urinary 8-hydroxydeoxyguanosine (8-OHdG) DNA adduct has been used as a biomarker in epidemiological studies. However , the determinants for urinary 8-OHdG have not been clearly identified. We tested urinary 8-OHdG levels in 205 male workers who had been exposed to vinyl chloride monomer (VCM) . Epidemiological information was obtained by an interviewer -administered questionnaire. Hepatitis B surface antigen ( HBsAg) and anti-hepatitis C antibody (anti-HCV) were also determined by immunoassay. Plasma antioxidants including Vitamins A and E, alpha- and beta-carotenes were assayed by high performance liquid chromatography. Median of urinary 8- OHdG level was 9.8 ng/mg creatinine (range, 1.4- 60.1). Multiple linear regression analysis showed that alcohol drinkers had higher urinary 8-OHdG than those who did not, but there was no dose-response between the amount of alcohol consumption and urinary 8-OHdG. Workers with positive HBsAg , anti-HCV and elevated plasma Vitamin A level were independently associated with higher levels of urinary 8- OHdG, whereas age, smoking, body mass index, plasma alpha- and beta-carotenes, Vitamin E levels, or VCM exposure did not show such an association. The results suggest that active inflammation of hepatitis B and C, alcohol consumption and higher Vitamin A level can induce oxidative stress. Thus, we conclude that potential determinants need to be considered in epidemiological studies when urinary 8- OHdG is used as a biomarker. (C) 2002 Elsevier Science B.V. All rights reserved.
Subjects
OXIDATIVE DNA-DAMAGE
VINYL-CHLORIDE
BETA-CAROTENE
HEPATOCYTES
RETINOL
SMOKING
SDGs
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
36.pdf
Size
70.65 KB
Format
Adobe PDF
Checksum
(MD5):8a99b88cfe91adbcb029bfd319760e9b