Caulogenesis and Somatic Embryogenesis in Bulblet Section Culture of Spider Lily Dikaryotype Hybrids (Lycoris aurea x L. radiata.)
Date Issued
2008
Date
2008
Author(s)
Wang, Lein- Han
Abstract
Lycoris aurea is the native summer flowers in Taiwan. It’s beautiful cut flowers of good quality and can adapt to Taiwan’s hot weather. It’s a great potential for development of summer flowers. They require four to five years from seed to flower, and propagation rate is restrained. This study focused on how to speed up the breeding of Lycoris hybrid.Experiment material is Lycoris dikaryotype hybrid E-3-3 x LRK4. Using adventitious bud originated from inflorescence to be the initiatory explants, and cut by cross then cultured on the media to induce. Using mind auxin (NAA, IAA) or PGR-free medium induced direct organogenesis and a few callus formed. Foe example cultured on the MS medium supplemented with IAA 2 mg/L + BA 2 mg/L the regeneration rate of adventitious buds is 60%, but callus formed only 11%. Using strong auxin (2,4-D, Picloram and Dicamba ) induced indirect organogenesis and somatic mebryogenesis. Like MS medium supplemented with NAA or IAA 2mg/L+BA 2 mg/L+2, 4-D 1mg/L can induce more embryogenic callus. Dark-Light treatment is no significant impact on induced callus, and the rates are higher then 54%. More embryogenic callus formed on N6 medium with 2,4-D 1 mg/L and BA 2 mg/L. The dedifferentiation rates of callus were 100% and the regeneration rate of somatic embryo were 55%. Higher concentration (2 mg/L) of 2,4-D can make abnormal embryos occurred, and lower concentration (0.1-0.5 mg/L) induced less effective.sing temporary immersion system (TIS) speeds up the breeding of Lycoris scales adventitious buds, compared with the solid medium reproduction rate. To propagation the shoot for one generation on TIS could four-weeks-shorter then on solid medium. The but proliferation was induced on MS medium supplemented with NAA 2 mg/L and BA 10mg/L, results indicated 45.3 buds under 15 min/3hr immersion time. After culture in liquid medium with 90 g/L sucrose 4 weeks, the bulblet grew well and the survival rate of plantlet was high.n cell suspension culture, embryogenic callus cultured in liquid N6 medium with 2,4-D 1 mg/L. After 6 months subculture, the cells can be observed for the sand-like appearance, most of the cytoplasm of cells thick, round or oval shape for the shape of the cell mass. Extracellular pH change values from 4.7 start training gradually increased to 6.0-6.25.dd pH buffer effect the formation of cells and extra cellular pH change. Suspension cells cultured in liquid N6 medium with 2,4-D 1 mg/L and MES (2-N-morpholino-ethanesulfonic) 10 g/L, and controlling extracellular pH at 6.3 before autoclave. After cultured, parked cell volume is 0.3 mL better then others, and extracellular pH can maintain stability in around 6.3.ycoris suspension cell culture plate tests, the treatments including plating composed of medium, add pH buffer, MES, pretreatment cells and regeneration medium and classified according to size before plating. Only the larger cell mass (> 15 mash) can be observed that the proliferation, but not further observed that the cell mass of renewable adventitious buds or somatic embryos.
Subjects
Lycoris
cell suspension culture
temporary immersion system (TIS)
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R95628125-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):5f190e736b592ae0d76b518dbc563d0d