尋找與人類肝癌有關的抑癌基因之研究
Date Issued
2001
Date
2001
Author(s)
許金川
DOI
892323B002011
Abstract
Hepatocellular carcinoma (HCC) is one of the most common cancer in the world and is the
leading cause of cancer death in Taiwan. The prognosis of this cancer is extremely poor with
survival of only several months after symptoms occurred. Elucidation of the basic genetic changes
of HCC is important for the understanding and treatment of this cancer. Cancer is usually
accompanied with genetic alternations either through the activation of cellular oncogene or the
inactivation of cancer suppressor gene. The recently identified short tandem repeat, the
microsatellite, which is widely distributed throughout the whole human genome. Identification of
disease genes as well as tumor suppressor genes by microsatellite polymorphism. have been
published recently.
In this study, we have used 35 microsatellite markers for further fine mapping of LOH. We
have confirmed the most frequent regions of LOH for HCC are 16q12.1, 16q22, and 16q24. After
analyzing these information, we started to screen the human BAC(Bacterial Artificial
chromosome)library by these markers at 16q12.1 and we identified 15 clones. Exon trapping system
is used to search the putative exon sequences of BAC genomic clones. Two exon-like sequences are
identical to the KIAA1005 gene. Homozygous deletion of KIAA1005 was found in 37%(10 /27)
HCCs. These data suggested that the KIAA1005 might be the putative tumor suppressor genes at
chromosome 16q12.1.
In microarray study, we have assayed 4 pairs of HCCs patients to compare differential gene
expression between nontumor part and tumor part. Of the informative genes, 0.3%-0.6% had an expression level in cancer that was 2 fold lower or higher than that in the non-tumor liver samples ,
designated as differentially expressed genes. The clustering analysis is in process in our lab. As for
these up-regulated or down regulated genes, we will apply quantitative PCR to prove these
differences in HCCs.
Subjects
hepatocellular carcinoma
micorsatellite analysis
tumor suppressor gene
SDGs
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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