Pneumatic Transfer Molding to Fabricate Three-Dimensional Biodegradable Porous Scaffolds
Date Issued
2012
Date
2012
Author(s)
Lin, MING-TUNG
Abstract
Porous 3D biomedical scaffolds have been widely used in tissue engineering. The scaffold can be used to culture autologous cells in vivo, stimulated by growth factors and signals. The cells can grow into the desired tissue morphology. Finally, it can be transplanted back into the body, through the stable tissue growth achieving the purpose of tissue repair. Conventional scaffolds are mostly prepared by organic solvents or rapid prototyping. There are problems such as residual solvent and complicated process. In order to solve these problems, a pneumatic transfer molding machine is designed and implemented to fabricate scaffolds in this study.
We first mold the disk biomedical scaffolds. It is found that the best weight ratio of PLGA:NaCl is 1:11. The experimental result also shows the best forming parameters are 90℃ for mold temperature, 200℃for the tube heating temperature and 4 kgf/cm2 for gas pressure. The scaffolds after particulate leaching yield porosity over 90% with pore size 200 ~ 500 μm, which satisfies what Health Medical chondrocytes need. Furthermore, the narrow version of the human-ear-shaped scaffold is also molded in this research. The mold is fabricated by rapid protyping and then by casting using PDMS. The transfer moldings of PLGA/NaCl using PDMS mold, after leaching, can yield ear-shaped scaffolds with pore size 200 ~ 500 μm.
The disk scaffold is used to culture cartilage cells. The morphology and distribution of the chondrocytes have been examined by fluorescence confocal microscopy and SEM. The cell growth behaviors has been determined by MTT assay, collagen, and glycosaminoglycan (GAG) secretion. After culture for 1 day, 84% of cartilage cells attached on scaffold. This proves that the transfer molded PLGA/NaCl, after leaching, can yield scaffolds with interconnected pores for culturing cells.
Subjects
Pneumatic Transfer Molding
Porosity
Mositure
Scaffold
Cell
Cartilage
SDGs
Type
thesis
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