內泌素對山羊黃體細胞株細胞凋亡及類固醇生成調控之研究
Date Issued
2000
Date
2000
Author(s)
DOI
892313B002064
Abstract
The purpose of this study was
determine the effects of hormones on
progesterone production and apoptosis
induction of carprine luteal cell line by
using in vitro strategy. Furthermore, to
examine the hormone effect on life span of
corpus luteum and ability of
steroidogenesis. In this study, we use a
caprine luteal cell line (GLC-D) with
temperature sensitive SV40 virus, which
established in our laboratory. In 40 ℃
culture system, we found that the treatment
of estrogen(100ng/ml) for 24 hours can
induce apoptosis of this cell line, and
decrease progesterone production from this
cell line. The inhibition effect of E2 may
include inhibition steroidogenesis enzyme
synthesis. In conclusion, the results
demonstrated that the inhibition of
steroidogenesis could induced apoptosis of
carpine luteal cell line, and increase DNA
fragmentation and 3’end labeling DNA
fragment , the mechanism is not clear yet
and further studies will be done.
Subjects
goat
cell line
apoptosis
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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