Effects of Glucosamine on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Cells
Date Issued
2012
Date
2012
Author(s)
Mao, Chien-Nong
Abstract
Glucosamine is monomer of chitosan, and an kind of amnio-monosaccharide. It is also be called 2-amino-2deoxy-glucose，and is the NH2 group substituted the H2O group of glucose。Some studies reported glucosamine can up-regulate the gene expression in chondrocyte, minimize degradation of cartilage matrix, and supply the source for synthesis of aggrecan. Other studies reported glucosamine can down-regulation some inflammatory cytokines, like COX-2、IL-1β、TNF-α, and also can promote differentiation of osteoblast. In order to investigate if glucosamine can be applied for oral medicine, we cultured human periodontal ligament cells and design different groups of experiments with the supplement of glucosamine or not. We check the ALP activity, the osteogenic-related gene expression, and the extracellular matrix mineralization in glucosamine-treated PDL cell cultures, and investigate the effects of glucosamine on osteogenic phenotype of DL cells.
The results of our study reveal that : Glucosamine can significantly promote the cell proliferation of PDL cells. In the presence of 5ug/ml glucosamine cultured, Runx2 gene of PDL cells is up-regulated, whereas this effect decreased with the culture time. ALP gene expression is up-regulated after 7 days of culture. The presence of 5ug/ml glucosamine in regular medium for 7 and 14 days did not induce the production of mineralized nodules in PDL cells cultures. When mineralizing medium was used, glucosamine can promote the osteogenic differentiation of PDL cells and accelerate the formation of mineralized nodules.
Subjects
Glucosamine
Human periodontal ligament cell
Osteogenic differentiation
SDGs
Type
thesis
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