Preparation, Characterization and Application of Porius Chitosan Microparticles
Date Issued
2007
Date
2007
Author(s)
Hung, Wei-Hsiang
DOI
zh-TW
Abstract
Abstract
In this research, porous chitosan microparticles were prepared by using a
emulsification/freeze-gelation method. The effects of various processes
parameters were investigated. The BSA-loading capability of the porous chitosan
microparticles and that of a commercial powder were compared.
First, the optimum HLB value of the surfactant utilized in this method was
found to be in the range of 8.6-11.0. The size of the porous chitosan
microparticles became smaller as the HLB values of the different surfactants
approached the optimum HLB value. These microparticles prepared by using
different surfactants had different structures；some were dense porous and some
were loose porous. Second, Span 20/Tween 21 was found to be the optimum
combination of surfactants with the HLB value of 10. The microparticles had
homogeneous and loose porous structure, and the particle size was 153.4 μm and
C.V. was 33.9%. Third, in the emulsification system when the proportion of
petroleumbenzin (mL) / 1 wt% chitosan solution (mL) / surfactants (g) was adjusted to
100/20/1.5, the particle yield was increased by 3.2 folds with size of 163.3 μm (C.V. =
37.3%). Fourth, this research also investigated the influence of freezing temperature.
The water uptake capability of microparticles increased obviously when the freezing
temperature decreased. The water uptake capability was about 2300% when the freezing
temperature was -25℃, which was 5.5 folds of that of commercial powder. Finally, the
application of the porous chitosan microparticles in BSA loading indicated that BSA
loading was complete at low BSA concentration (＜0.8 mg/mL) and was 190 mg
BSA/g chitosan at high BSA concentration (＞2.0 mg/mL). The loading capacity of
these microparticles was 4 folds of that of commercial powder.
This research demonstrated that the properties of the porous chitosan
microparticles could be modulated by varying the process parameters for
application in various fields.
Subjects
幾丁聚醣
多孔性
Chitosan
Porous
Type
thesis
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