Search of mating type-related genes of Phytophthora parasitica by amplified fragment length polymorphism
Date Issued
2006
Date
2006
Author(s)
Lai, Chong-Cheong
DOI
zh-TW
Abstract
Phytophthora parasitica is an important plant pathogen with a broad host range capable of infecting more than 72 plant genera. P. parasitica is heterothallic with A1 and A2 mating types have been isolated in Taiwan. The sexual reproduction of Phytophthora plays an important role not only in genetics exchange, but also as a survival structure. In spite of numerous efforts made to determine the mating type of Phytophthora not much is known at the molecular level. Retroposon-base DNA fingerprint of 98130/A1 and 98130/A2 isolates of P. parasitica were analyzed.The hybridization pattern of 98130/A1 was almost the same as 98130/A2 with the exception of few bands, indicating that these two strain were isogenic. In AFLP experiment, a total 32 AFLP primer sets were analyzed for their ability to distinguish isolates of opposite mating type. Although the AFLP patterns were similar in most cases, 8 bands were found only in one mating type but not the other. Sequence analysis showed three of to containe homologous sequences of Rumex acetosa Y chromosome specific tandem repeat however, further study is needed to confirm its genetic link to mating type. In cDNA-AFLP experiment, a total of 40 combination primer sets were analyzed for their ability to distinguish between 98130/A1 and 98130/A2 in stable environmental conditions. Although the cDNA-AFLP patterns were similar in most cases but there did exit 44 differential transcription derivate fragments. The transcription derivate fragment A15T13-2 revealed by cDNA-AFLP expressed higher in 98130/A2 than 98130/A1. The validity of this result was comfirmed by northern hybridization and real-time PCR. The performance of northern hybridization with increased number of A1 and A2 isolates showed similar result, indicating that the phenomenon of higher expression of A15T13-2 in A2 isolates of P. parasitica is universal. A15T13-2 showed high similarity with Danio rerio aquaporin3 by sequence analysis. These differential transcription derivate fragments need further study to confirm its linkage with either mating type determination or mating process.
Subjects
疫病菌
增幅性片段長度多型性
Phytophthora parasitica
amplified fragment length polymorphism
Type
other
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