Development of a flow-injection liposome immunoanalysis system for fumonisin B1
Journal
Analytica Chimica Acta
Journal Volume
414
Journal Issue
1-2
Pages
61-69
ISSN
0003-2670
Date Issued
2000-06-01T03:26:58Z
Author(s)
Durst, R.A.
Abstract
Fumonisins are a group of naturally occurring mycotoxins produced by several species of the fungus Fusarium (mainly moniliforme) and occur worldwide on corn intended for human and animal consumption. Contamination of food and feed with fumonisins has been implicated in and associated with a number of diseases in both livestock as well as human beings. In this study, a Flow-Injection Liposome ImmunoAnalysis (FILIA) system was developed for the heterogeneous quantitative determination of fumonisin B1 (FmB1). The immunoassay is based on the competition between FmB1 and sulforhodamine B (SRB) dye-encapsulating, FmB1-tagged liposomes for a limited number of antibody binding sites. The antibodies are immobilized via protein A in a capillary immunoreactor column, and 35% MeOH is used for the regeneration of antibody binding sites after each measurement, which allows the immunoreactor to be used for up to 70 sequential sample injections without any loss of reactivity. Sample preconcentration is not needed and a single assay can be performed in less than 11min. The calibration curve for FmB1 in Tris-buffered saline (TBS) solution had a working range of 1-1000ng/ml.
Subjects
Biosensors
FILIA
Flow-injection liposome immunoanalysis
Fumonisin B1
Immunoassay
Liposome
Mycotoxin
Publisher
Elsevier Science B.V.
Type
journal article