Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei 01
Journal
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Journal Volume
721
Journal Issue
2
Pages
157-162
Date Issued
2011
Author(s)
Abstract
Cell fractions including heat-treated cells, crude cell walls, intracellular extracts and exopolysaccharides (EPSs) obtained from Lactobacillus casei 01 were first studied for their effects on the proliferation of human intestinal epithelial cells, intestine 407 and the human colon cancer cell, HT-29. Their effects on the cytotoxicity of 4-nitroquinoline 1-oxide (4-NQO) against intestine 407 were further investigated. The results revealed that EPS exhibited the highest antiproliferation activity on HT-29 cells while the viability of intestine 407 cells was not affected by EPS at a concentration of 5-50 μg/mL. It was also noted that all the cell fractions and EPS from L. casei 01 reduced the cytotoxicity of 4-NQO against intestine 407 with EPS showing the highest anticytotoxic activity. Additionally, it was found that EPS might exert blocking and bioanticytotoxic effects by both adjusting the function of intestine 407 and repairing the 4-NQO-damaged cells, thus reducing cytotoxicity of 4-NQO. ? 2011 Elsevier B.V.
Subjects
4-Nitroquinoline 1-oxide; Anticytotoxicity; Comet assay; Exopolysaccharides; Lactobacillus casei 01
SDGs
Other Subjects
4 nitroquinoline 1 oxide; antimutagenic agent; cytotoxic agent; exopolysaccharide; probiotic agent; antineoplastic activity; article; bacterial strain; cell fractionation; cell function; cell proliferation; cell strain HT29; cell viability; concentration response; controlled study; DNA repair; drug cytotoxicity; drug effect; human; human cell; intestine epithelium cell; Lactobacillus casei; mutagenicity; priority journal; 4-Nitroquinoline-1-oxide; Antimutagenic Agents; Cell Proliferation; Cell Survival; HT29 Cells; Humans; Intestines; Lactobacillus casei; Mutagens; Polysaccharides, Bacterial; Lactobacillus casei
Type
journal article