5-氨基酮戊酸光動力治療誘導第二型 環氧化酶表現之調控機轉
5-ALA Photodynamic Therapy induce the Up-regulation of COX-2 in oral cancer cells
Date Issued
2007
Date
2007
Author(s)
Chen, Po-Chang
DOI
zh-TW
Abstract
Oral cancer is the fourth leading cause of cancer-related deaths in male population in Taiwan. Despite recent advances in radiotherapy and chemotherapy, the survival of patients with oral cancer has not improved significantly. Continued investigation of new chemotherapeutic agents is thus needed. Recently, 5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has been used to treat oral premalignant and malignant lesions with promising results in our clinical trials. However, previous studies showed that PDT induces the expression of proangiogenic factors, such as cyclooxygenase-2 (COX-2), thereby promoting cancer cell regrowth following PDT. In this study, we show ALA-PDT can also increase COX-2 expression. Pretreatment of Ca9-22 cells with specific inhibitors of Phosphatidylinositol-specific phospholipase C (PI-PLC; U-73122), protein kinase C (PKC; Go6983) and NF-κB (Bay 11-7082) inhibited COX-2 induction. In summary, ALA-PDT induces the COX-2 expression through the cascade of PI-PLC/PKCβ/NF-κB. Furthermore, we found ALA-PDT increased the expression of Cyr61. Pretreatment of cells with Cyr61 shRNA inhibited ALA-PDT induced COX-2 expression. To our knowledge, this is the first time to show PDT can induce Cyr61 expression and thereby promoting cancer cell regrowth following PDT.
Subjects
5-氨基酮戊酸
光動力治療
第二型環氧化酶
5-ALA
Photodynamic Therapy
COX-2
SDGs
Type
other
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