Raman Spectrospic Analysis of the Knee Cartilage
Date Issued
2007
Date
2007
Author(s)
Chang, Hong-Wen
DOI
zh-TW
Abstract
Osteoarthritis is a degeneration disease that results from corroded or abraded cartilage which acts as buffering cushion. In this study, we set up an analytic system using Raman Spectrum as measuring tool. First, animal's articular cartilage was used as test sample, and the material properties were assessed using a microscopic Raman Spectrum. Then, a specially designed compressing relaxation measurement system was adapted to the Raman spectrum apparatus to test mechanical properties of the cartilage. The aim was to study the influence various loading stresses on the knee joint cartilage deformation and Raman spectrum. The degenerated cartilages of pig were used as animal model for osteoarthritis verifications. Finally, in vitro osteoarthritis human study was conducted to test the data analysis procedure and the clinical relavency.
The scanning results of the cross section of articular cartilage suggest that the 1350 cm-1 and 462 cm-1 can be as the Raman markers for cartilage study. We have shown the feasibility of mechanical property study of cartilage using of Raman spectrum-scopy and loading-unloading stress relaxation measurement system. The joint cartilage material properties changed during loading and unloading procedure, and the nonlinear viscoelastic behaviour was observed. The Raman spectrum analysis for the diagnosis of osteoarthritis has been tested in degenerating pig and human cartilages, and the results show that the two characteristic indicators are located in the amide III envelope (1300-1350 cm-1) and skeletal modes (400-500cm-1).
Subjects
拉
曼光譜
膝關節軟骨
退化性關節炎
機械特性
膠原纖維
蛋白聚醣
應力鬆弛
Raman spectroscopy
Cartilage degeneration
Osteoarthritis
Stress relaxation
SDGs
Type
thesis
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