The effects of Se-allylselenocysteine (ASC), an analogue of garlic compound, on DMBA/TPA-induced skin tumorigenesis
Date Issued
2015
Date
2015
Author(s)
Jiang, Wen-Ru
Abstract
Se-allylselenocysteine (ASC), an analogue of garlic compound, has been shown to inhibit mammary carcinogenesis in vivo and cell growth in vitro. However, the function of ASC on anti-inflammatory effect remains largely unknown. Therefore, we investigated whether ASC has anti-inflammatory on LPS-induced inflammation in vitro or anti-tumor promoting on 7, 12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin tumorigenesis in vivo and tried to elucidate the mechanisms involved. In in vitro study, the result showed that ASC inhibited lipopolysaccharide (LPS)-induced production of nitric oxide (NO) with decreased protein level of inducible nitric oxide synthase (iNOS) in RAW 264.7 cells. ASC also reduced nuclear factor-B (NF-B) luciferase activity. On the other hand, ASC can enhance LPS-induced COX-2 protein level and mRNA expression in RAW 264.7 cells. In in vivo study, topical application of ASC on the dorsal skin of DMBA-initiated and TPA-promoted mice could significantly accelerate skin tumorigenesis and raise tumor multiplicity than positive control group (DMBA+TPA). The number of tumors that were 1–3 mm, 3–5 mm and >5 mm in size per mouse was increased in a dose-dependent manner in the ASC-pretreated groups. Pretreatment with ASC showed significant increment on the expression of COX-2 compared with positive control group. In summary, the present results speculate that COX-2 played a crucial role in tumor-promoting effect of ASC on DMBA/TPA-induced skin cancer in mice.
Subjects
anti-inflammation
Se-allylselenocysteine (ASC)
iNOS
COX-2
DMBA/TPA-induced skin tumorigenesis
SDGs
Type
thesis
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