Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells
Resource
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 1, 9
Journal
Evidence-based Complementary and Alternative Medicine
Pages
1
Date Issued
2011
Date
2011
Author(s)
Lu, Tzu-Li
Chuang, Jing-Yuan
Yang, Jai-Sing
Chiu, Shau-Ting
Hsiao, Nai-Wan
Wu, Mei-Chen
Wu, Shih-Hsiung
Hsu, Ching-Hsiang
Abstract
Type-2 ribosome-inactivating proteins, composed of a toxic A-chain and lectin-like B-chain, display various biological functions, including cytotoxicity and immunomodulation. We here cloned the lectin-like B-chain encoding fragment of a newly identified type-2 RIP gene, articulatin gene, from Viscum articulatum, into a bacterial expression vector to obtain nonglycosylated recombinant protein expressed in inclusion bodies. After purification and protein refolding, soluble refolded recombinant articulatin B-chain (rATB) showed lectin activity specific toward galactoside moiety and was stably maintained while stored in low ionic strength solution. Despite lacking glycosylation, rATB actively bound leukocytes with preferential binding to monocytes and in vitro stimulated PBMCs to release cytokines without obvious cytotoxicity. These results implicated such a B-chain fragment as a potential immunomodulator.
Type
journal article
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