Continued monitoring of De Novo Lipogenesis by Tissue Engineering
Date Issued
2002-07-31
Date
2002-07-31
Author(s)
湯月碧
DOI
902314B002414
Abstract
The main purpose of this experiment is using gene transfection in the
tissue engineering about adipogenesis. In the previous study, we used
basement membrane complex (Matrigel or Cymetra) mixed with biodegradable
gelatin microspheres, a controlled drug release system, was injected into
subcutaneous layer of mice. bFGF, growth hormone and IGF-1 were
incorporated into these microspheres respectively before mixing. The
adipose precursor cells are fibroblast like precursor cel ls and could be
stimulated by growth hormones to migrate into the scaffold and
differentiate to mature fatty tissue. Although this drug releasing system
could induce adipogenesis, the growth factors were released continuously
but could not be stopped or restarted releasing at any time as we will.
If we transfect genes of growth factors we need with regulatory genes into
adipocyte precursor cells or other mesenchymal cells such as fibroblasts,
the desired growth factors could be released stably and the genes could
be turned on or off at any time we need. The cationic lipid reagents are
the transfection system of our choice because it yields high transfection
efficiency in a wide variety of eukaryotic cells, is simple to perform,
and ensures consistently reproducible results. Here in vitro studies by
transfection of desired genes into cultured cells were tried, if the
results are satisfactory, we will apply it further to in vivo studies.
Subjects
組織工程
脂肪組織增生
生長因子
基因轉殖
調控基因
正離子脂質體
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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