Tape-Stripping Electrochemical Detection of Melanoma
Journal
Analytical Chemistry
Journal Volume
91
Journal Issue
20
Start Page
12900
End Page
12908
ISSN
00032700
Date Issued
2019
Author(s)
Darvishi, Sorour
Pick, Horst
Zhu, Yingdi
Li, Xiaoyun
Ho, Ping-Chih
Girault, Hubert H.
Lesch, Andreas
Abstract
A noninvasive electrochemical melanoma detection approach based on using adhesive tapes for collecting and fixing cells from a suspicious skin area and transferring the cells into a scanning electrochemical microscope (SECM) is presented. The adhesive layer collects the cells reproducibly and keeps them well adhered on the tape during experiments in an electrolyte solution. A melanoma biomarker, here the intracellular enzyme tyrosinase (TYR), was imaged on the tape-collected cells without further cell lysing using antibodies that were labeled with horseradish peroxidase (HRP). The HRP labels catalyzed the oxidation of a dissolved redox-active species, which was detected at a soft microelectrode, gently brushed in contact mode over the tape. The melanoma biomarker was first detected on tape-stripped samples with murine melanoma cells of different concentrations. Thereafter, increasing levels of TYR were recorded in cells that were collected from the skin of melanoma mouse models representing three different stages of tumor growth. Additionally, SECM results of tape-stripped different human melanoma cell lines were confirmed by previous studies based on traditionally fixed and permeabilized cells. © 2019 American Chemical Society.
Publisher
American Chemical Society
Description
論文編號: 12900
Type
journal article