MINING THE GIARDIA LAMBLIA GENOME FOR NEW CYST WALL PROTEINS
Resource
JOURNAL OF BIOLOGICAL CHEMISTRY, v.278 n.24, pp.21701-21708.
Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Journal Volume
v.278 n.24
Pages
21701-21708
Date Issued
2003
Date
2003
Author(s)
孫錦虹
SUN, CHIN-HUNG
Abstract
The Giardia lamblia cyst wall ( CW), which is required for survival outside the host and infection, is a primitive extracellular matrix. Because of the importance of the CW, we queried the Giardia Genome Project Database with the coding sequences of the only two known CW proteins, which are cysteine- rich and contain leucine-rich repeats ( LRRs). We identified five new LRR- containing proteins, of which only one ( CWP3) is up- regulated during encystation and incorporated into the cyst wall. Sequence comparison with CWP1 and - 2 revealed conservation within the LRRs and the 44- amino- acid N- flanking region, although CWP3 is more divergent. Interestingly, all 14 cysteine residues of CWP3 are positionally conserved with CWP1 and - 2. During encystation, C- terminal epitope- tagged CWP3 was transported to the wall of water- resistant cysts via the novel regulated secretory pathway in encystation- secretory vesicles ( ESVs). Deletion analysis revealed that the four LRRs are each essential to target CWP3 to the ESVs and cyst wall. In a deletion of the most C- terminal region, fewer ESVs were stained in encysting cells, and there was no staining in cysts. In contrast, deletion of the 44 amino acids between the signal sequence and the LRRs or the region just C- terminal to the LRRs only decreased the number of cells with CWP3 targeting to ESVs and cyst wall by similar to 50%. Our studies indicate that virtually every portion of the CWP3 protein is needed for efficient targeting to the regulated secretory pathway and incorporation into the cyst wall. Further, these data demonstrate the power of genomics in combination with rigorous functional analyses to verify annotation.
Type
other