A SELF-CONTAINED PORTABLE POLYMERASE-CHAIN-REACTION SYSTEM INTEGRATED WITH ELECTROMAGNETIC MINI-ACTUATORS FOR BI-DIRECTIONAL FLUID TRANSPORT
Resource
JOURNAL OF MECHANICS, 27(3), 357-364
Journal
Journal of Mechanics
Pages
357-364
Date Issued
2011
Date
2011
Author(s)
Abstract
ABSTRACT In this paper, the development of a portable polymerase chain reaction (PCR) device is presented. Integrating electromagnetic mini-actuators for bi-directional fluid transport, the proposed device, whose dimension is 67mm × 66mm × 25mm, can be fully operated with a 5V DC voltage. The device consists of four major parts: A disposable channel chip in which PCR mixture is manipulated and reacted, a heater chip which generates different temperature zones for PCR reaction, a linear actuator array for pumping PCR mixture, and a circuit module for controlling and driving the system. The advantages of the device include the rapid temperature responses associated with continuous-flow-type PCR devices, as well as the programmable thermal cycling associated with chamber-type PCR devices. The thermal characteristics are measured and discussed. PCR amplification is successfully performed for the 122 bp segment of MCF-7/adr cell line. Due to its small footprint, this self-contained system potentially can be employed for point-of-care (POC) applications.
SDGs
Type
journal article
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