Process Design for the Effects of Biomaterials on Cellular Senescence
Date Issued
2009
Date
2009
Author(s)
Chang, Hsin-Chun
Abstract
Poly (ethylene-co-vinyl alcohol) (EVAL) is a copolymer which has been widely used as biomaterials recently. In our laboratory, it is proved that primary skin dermal fibroblasts cultured on EVAL biomembranes have significant influences on cell morphology, growth curve, and gene expression associated with senescence. However, the recent process of studying senescence phenomenon has some major shortcomings such as slow-rating division, long time for cultivation, and risks of pollution or poor cell conditions occasionally. In this research, the main purpose is to set up a quick-acting method, which can greatly shorten the cultivation time. Thus, the senescent effects by different biomembranes could be examined more effectively. Here, the Nasopharyngeal Carcinoma (NPC) cell line, HONE-1, and non-small cell lung carcinoma (NSCLC) cell line, H1299, are used as experimental cell to observe the behaviors of senescence. In this manner, the replicative senescence is avoided due to the rapid cleavage rate of the cell line. On the other hand, because of the lack of p53 gene, the cell line H1299 may go to senescence in another pathway different from that originally involved in p53 gene. In the point of view, the effect of gene p53 on cell senescence can also be discussed. Besides, in the experiment, the cell is cultured on three types of surface; they are EVAL44 (EVAL which composed of 44 mol% hydrophobic ethylene segment), EVAL27 (EVAL which composed of 27 mol% hydrophobic ethylene segment), and TCPS (tissue culture polystyrene plates), respectively. The experimental procedure is classified into three modes. One mode is that the cells are cultured on the three types of biomembrane surefaces for a long time to distinguish the differences among them. Another one is that the cells are cultured on the three biomembrane surfaces and exposed to ethanol at the same time. The other mode is that the cells are cultured on the three biomembrane surfaces after exposure to ethanol for several times. After that, the senescence effects on the cell line by the different biomembranes were observed. As shown in the results, however, the three types of surface have limited influence on the cancer cell line. If the cells were treated with ethanol, they may go to the senescent stage. Otherwise, if ethanol treatment was cancelled, the difference in division rate can hardly be observed, but the cells didn’t go to senescence. Therefore, the designed process is not as satisfactory as expected, for its inability to improve the long-time consuming problem.
Subjects
cellular senescence
polymer biomembranes
cell line
poly(vinyl alcohol-co-ethylene)
ethanol
SDGs
Type
thesis
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