Differentiation of basal cell carcinoma from the adjacent normal tissue by of non-linear microscopy:implication for surgical guidance
Date Issued
2006
Date
2006
Author(s)
Kuo, Chien-Jui
DOI
zh-TW
Abstract
Basal cell carcinoma is the most common skin cancer in the oriental population. It usually develops in the sun-exposed skin. Conventionally, the cancer is removed by wide excision, aimed at preventing local recurrence. When a wide safety margin can not be obtained in areas including eyelids, periorbital area and nasal alae, Mohs’ surgery is employed to ensure complete elimination of cancer cells while preserving these anatomically important structures. However, it takes time for tissue processing for microscopical examination. In this work, we use multiphoton fluorescence and second harmonic generation microscope to examine basal cell carcinoma specimens. The autofluorescence and second harmonic generation signals are compared with routine histological examinations using H&E staining. Our results show that cancer cells and caner stroma can be differentiated from the adjacent normal skin using multiphoton fluorescence and second harmonic generation microscopy.
Subjects
基底細胞癌
多光子顯微術
自發螢光
倍頻顯微術
Basal cell carcinoma
Mohs’ surgery
Multiphoton microscope
autofluorescence
second harmonic generation
SDGs
Type
thesis
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