Application of Dual-Channel CE-MS Interface in Protein Analysis
Date Issued
2007
Date
2007
Author(s)
Tzu, Cha-Chi
DOI
zh-TW
Abstract
Separation of protein digested peptides and amino acid sequencing has become a common practice for protein identification in proteomics. Currently, capillary LC/MS is the major technique used to identify the proteins separated by 2-D gel electrophoresis. One HPLC run takes about 60 minutes in analysis of the digested peptides. The
HPLC/MS approach suffers from low sequence coverage, because there are too many peptides in one LC peak. Recently, multiple injection CE/MS under the same run
utilizing the dynamic exclusion capability of the mass spectrometer has been reported to improve the sequence coverage. In this study, to improve sequence coverage,
dual-channel interface developed by our lab was used to combine two separation methods (such as CEC and CE), or two separation conditions (such as different pH) and the multiple injection for analyzing the tryptic digested peptides. By utilizing the two channel interface, there is no need to condition and wash the capillary when different
separation conditions were used. The results showed that after four injection and 35 min, sequence coverage can be improved to 85% in the analysis time of bovine serum albumin.
Subjects
蛋白質分析
毛細管電泳質譜
毛細管電層析質譜
多次進樣
雙通道界面
序列涵蓋率
protein analysis
CE-MS
CEC-MS
sequence coverage
dual-channelnterface
multiple injection
Type
thesis
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