Proteomic Analysis of Caco-2 Cells Treated with Monacolin K
Resource
Journal of Agricultural and Food Chemistry 54 (17): 6192-6200
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
54
Journal Issue
17
Pages
6192-6200
Date Issued
2006
Date
2006
Author(s)
Abstract
Monascus species is an important traditional fermentation fungus used on food. Monacolin K (a secondary metabolite of Monascus, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase) in inhibition of mevalonate synthesis may result in reductions of isoprenoid prenylation in cells. Impairment of protein isoprenoid prenylation has been related to anticancer effect in cancer cells. As a functional food for Monascus, however, the molecular mechanisms responsible for the anti-proliferate effect of monacolin K in cancer cells are not clear. We used proteomic analysis by two-dimensional gel electrophoresis, matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS), tandem mass spectrometry (MS/MS), and database interrogation to separate and identify the proteins of Caco-2 cells treated with monacolin K. The results showed that monacolin K inhibited the proliferation of Caco-2 cells in a dose-dependent manner. The identified proteins in proteomic analysis included anti-oxidation enzymes related to reactive oxygen species stress, cytoskeleton proteins, glycolytic enzymes, and enzymes involved in mediating protein interactions. Furthermore, glutathione S-transferase P 1 and cytoskeleton-8, -18, and -19 revealed a down-regulation in a dose-dependent manner in exposure of Caco-2 cells to monacolin K. ? 2006 American Chemical Society.
Subjects
Anti-cancer; Monacolin K; Monascus; Proteomics
SDGs
Other Subjects
antineoplastic agent; mevinolin; tumor protein; article; cell division; cell strain CACO 2; cell survival; chemistry; colon tumor; dose response; drug effect; gene expression; genetics; human; mass spectrometry; pathology; protein processing; proteomics; two dimensional gel electrophoresis; Western blotting; Antineoplastic Agents; Blotting, Western; Caco-2 Cells; Cell Division; Cell Survival; Colonic Neoplasms; Dose-Response Relationship, Drug; Electrophoresis, Gel, Two-Dimensional; Gene Expression; Humans; Lovastatin; Neoplasm Proteins; Protein Isoprenylation; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Fungi; Monascus
Type
journal article
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