Rapid and Specific Detection of Important Diseases of the Anguillid Eel by Polymerase Chain Reaction (PCR)
Date Issued
2014
Date
2014
Author(s)
Chang, Ya-Fen
Abstract
The anguillid eels are the most important freshwater fish in the aquaculture industry in East Asia and Europe (Joh et al., 2013). Since 1970s, because of the high price of glass eel and decrease of stock size (Han et al., 2010), some eel farmers have tried to culture other eel species. Unlike Anguilla japonica and Anguilla anguilla, there are a few aquaculture information about other anguillid eel. Moreover, diseases, include bacterial and parasites infection, are a major cause of economic loss in the aquaculture industry. For this purpose, the aim of this study is to develop a rapid and specific detection of important diseases of the eel. After designed specific primer sets, the optimal PCR conditions were determined. In addition, sensitivities were estimated to be as low as 10~1 ng for the genomic samples. Moreover, the PCR method was used for identified diseased sample and showed the diagnosis of accuracy. In conclusion, the assay described in this study is a sensitive and specific method for simultaneous detection of eight pathogens in eels.
Subjects
鰻鱺屬
養殖疾病
PCR檢測
專一性
敏感性
Type
thesis
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