A novel method for rabbit stifle joint cartilage regeneration with injectable platelet-rich fibrin releasate and bone marrow stem cell
Date Issued
2014
Date
2014
Author(s)
Tseng, Chia-Chuan
Abstract
Abstracts
Osteoarthritis, is a degenerative joint disease accompanied with non-inflammatory articular cartilage lesion, losing of extracellular matrix and develop into cracks, erosion, subchondral sclerosis, osteophytes and loss of joint function eventually. Unlike other connective tissues, articular cartilage does not contain blood vessels so it does not have the ability to regenerate and repair; therefore, materials that promote articular cartilage regeneration are important in tissue engineering studies.
Mesenchymal stem cells (MSCs) can be found and isolated in different tissue including bone marrow, peripheral blood, umbilical cord blood, fat, synovial capsule, periodontal ligament, skin or solid mesenchymal tissues. The ability to transform into connective tissue system including hard bone, cartilage, fat and muscle tissue, which were cultured in several conditions that may induced to different tissues.
Furthermore, functions of the platelets in medical research are not only coagulation and hemostasis, in the process of tissue repair and regeneration, the platelets can help regulate body metabolism, and promote tissue repair and healing. With the development of technology, we can get the concentrated platelet, platelet-rich fibrin releasate , which contains a variety of growth factor that maybe promote proliferation and differentiation of chondrocytes.
In this study, an injectable platelet-rich fibrin releasate combine bone marrow stem cells was used for articular cartilage regeneration. New Zealand white rabbits were divided into four groups for treatment (n=6 per group): (1) control group; (2) injected platelet-rich fibrin releasate 1ml; (3) injected platelet-rich fibrin releasate 0.8 ml combine bone marrow stem cells 3×106 cells/0.2 ml medium; (4) injected bone marrow stem cells 3×106 cells/1 ml medium. After fourteen weeks, the bone appearance of healing was evaluated including coverage, color, cartilage surface defect edge and surface smooth. Histological investigation including cartilage surface, matrix, cell distribution, cell population viability, subchondral bone and cartilage mineralization. A statistically significant difference between the experimental and control groups was observed. The third group closest to the normal cartilage tissue structure, then followed by the second group. It was concluded that the promising experimental data are beginning to emerge in support of the use of platelet-rich fibrin relesate combine bone marrow stem cells for regenerative applications.
Subjects
可注射性富含血小板纖維素釋放液
骨髓幹細胞
組織工程
關節軟骨再生
Type
thesis
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