Immunological, anti-tumor and antioxidant activities of phycocyanin from marine Synechococcus sp.
Date Issued
2006
Date
2006
Author(s)
Hsu, Hui-Chun
DOI
zh-TW
Abstract
Phycocyanin, one of the major biliproteins from blue green algae, has various biological and pharmacological properties. It is a water-soluble and non-toxic fluorescent protein pigment. The present study used two-step column chromatographic method to isolate and purify c-phycocyanin from a marine Synechococcus. The isolation involved a multi-step treatments of the crude extract by fractional precipitation with ammonium sulfate, then by Q-sepharose column and finally ceramic hydroxyapatite column, giving a purity ration A620/A280 of 4.9. The yield of c-phycocyanin was 35% from Synechococcus dried powder. Respectively, further the purity and homogeneity were confirmed by native and SDS-PAGE electrophoresis. The estimated molecular weight of the phycocyanin was 128 kDa as determined by Sephadex S-200 gel filtration and electrophoresis.
Phycocyanin from the reported alga was assayed in vitro for proliferation stimulation in murine macrophages and for anti-tumor activity against human mammary and pancreas tumor cells, using MTT method. The results indicated that macrophage proliferation increased by 38% while treated with phycocyanin at the concentration of 65 μg/ml and the stimulation activity was a dose-dependent manner. Tumor cells used in the present study reduced markedly in growth rate in the presence of phycocyanin, i.e. reduced by 80% at concentration of 65 μg/ml. The obtained phycocyanin was also an antioxidant as determined by DPPH method. Its antioxidation activity, as expressed in % scavenge of oxygen radicals, reached 36% at concentration of 78 μg/ml. The above findings revealed that marine Synechococcus and the extracted phycocyanin can be a potent source for biomedical research, as well as therapeutic agent not only in immune stimulation and modulation, but also in tumor growth inhibition.
Subjects
聚球藻
藻藍素
免疫
抗癌
抗氧化
Synechococcus
phycocyanin
immunological
anti-tumor
antioxidant activities
SDGs
Type
thesis
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