蛻皮激素對德國蜚蠊與雙紋姬蠊之活動行為日週律動的影響(2/3)
Date Issued
2004
Date
2004
Author(s)
DOI
922313B002054
Abstract
The expression of locomotor circadian
rhythm in female German cockroach is
masked by the development of ovaries.
Since the masking effect is mediated through
the regulation of hormone, we propose the
ecdysone, the major hormone secreted from
ovaries, is the masking factor. Firstly, by
analyzing the ecdysone receptor (EcR) gene
to realize ecdysone how to influence the
locomotor circadian rhythm, because
ecdysone will control the EcR gene which
influence expression of the gene on the
downriver. Firstly, using the antibody from
Lepidoptera to work the western bloting. In
addition, we will employ immunostain
method against ecdysone receptor to reveal
the action sites. These sites may shine light
on the interference of circadian signals with
masking factor-ecdysone. In order to prove
the masking role of ecdysone,
ovariectomized females who showed
unmasked locomotor circadian rhythm will
be treated with ecdysone analog to regain the
masked rhythm. Finally, protein sequencing
of EcR was done. Since the sibling species
of the Germany cockroach, the
double-striped cockroach is a feral species
and expresses locomotor circadian rhythm in
virgin females, it is a great advantage to use
this species as a comparative study to further
demonstrate the function of ecdysone on the
locomotor circadian rhythm. The same
experimental procedures, therefore, will be
repeated on the double-striped cockroach.
The main focus of this proposal is to
investigate the physiological mechanism of
masking effect on the locomotor circadian
rhythm by ecdysone.
Subjects
Germany cockroach
double-striped cockroach
locomotor circadian rhythm
masking effect
ecdysone
Publisher
臺北市：國立臺灣大學昆蟲學系暨研究所
Type
report
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