Involvement of a Novel C-Terminal Kinase Domain of Kir6.2 in the K-ATP Channel Rundown Reactivation
Date Issued
2000
Date
2000
Author(s)
Lou, Kuo-Long
DOI
892314B002258
Abstract
Rundown is a generally encountered problem while recording KATP
channel activity with inside-out patches. No assigned structural fragment related to
this mechanism could be derived from any of the proceeded functional analyses as yet.
Therefore, based on a combined sequence and secondary structure alignment against
known crystal structure of segments from closely related proteins, we propose here
the three-dimensional structure model of an intracellular C-terminal domain of the
Kir6.2 subunit in KATP channels. An E. coli CMP-kinase was suggested as template for
the model building. The subdomain arrangement of this novel kinase domain and the
structural correlation for UDP-docking were described. With structural-functional
interpretation, we conclude that the reactivation of KATP channel rundown by MgATP
or UDP is very possibly regulated by this intracellular kinase domain at the Cterminus
of Kir6.2 subunit in KATP channels.
Subjects
Channel gating
3-D homology modelling
Kinase domain
Publisher
臺北市：國立臺灣大學醫學院口腔生物科學研究所
Type
journal article
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