Effect of ultrasound induced gene transfection by chitosan/alginate nanoparticle
Date Issued
2008
Date
2008
Author(s)
Chang, Szu-Min
Abstract
Recently, several studies demonstrated that interaction of ultrasound with nanoparticles; perfluorocarbon nanoparticles, liposomes, cationic polymers (micelles, PEI, DOTMA etc.) and microbubbles, may enhance drug and gene delivery in vitro and in vivo. Here, we first introduced ultrasound to a chitosan-mediated gene delivery system, and investigated its effect on the transfection efficiency of this system. During ionic gelation, chitosan and pAcGFP1-C1 plasmid DNA spontaneously interacted to yield Chitosan-DNA nanoparticles (Chi-DNA NPs) with mean Z-average diameters range of 227–292 nm while Chitosan-alginate-DNA nanoparticles (Chi-alg-DNA NPs) had diameters range of 536–654 nm at various N:P ratio, which could potentially protect DNA from nuclease attack and provide beneficial properties for gene delivery. In 293T cells, the transfection efficiency could be enhanced by ultrasound up-to 30% in Chi-DNA NPs (at 3:1 & 5:1 N:P ratio) and 25% improvement of total transfected cells in Chi-alg-DNA NPs (at all ratio we tested). In comparison, transfection efficiency in Hela cells of our system was similar to Lipofectamine™2000. In sum, the combination of the two promising gene transfection strategies, chitosan-based nanoparticle and ultrasound-induced gene delivery system, could enhance gene transfection in vitro depending on the cell type with relatively minor cytotoxicity compared to the commercial liposome gene delivery agent.
Subjects
Ultrasound
gene transfection
chitosan nanoparticles
Type
thesis
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