Identification of Lcn2 gene expression in zebrafish and mouse brain
Date Issued
2010
Date
2010
Author(s)
Pan, Wei
Abstract
Lcn2 is a member of the lipocalin family and has been well known as an acute phase protein. Several evidences showed that Lcn2 is a multi-function protein, participating in such as immunity, apoptosis, ion transport, cell differenciation and proliferation. It may also be related to the occurrence of diseases. Subsequently, it has been found that the expression of Lcn2 gene triggered embryonic kidney development. The finding encourages us to characterize the Lcn2 expression in zebrafish and mice, and look for an animal model to elucidate the biological function of Lcn2. The mRNA level of Lcn2 in tissue distribution and stage profile of embryos in zebrafish and mice were analyzed by RT-PCR and Real-time PCR. Western blotting and immunohistochemical staining have performed for protein assay. The data showed that Lcn2 gene expression initiated in 10 hpf embryo and sustained till 72 hpf. Furthermore, with a mouse brain model, Lcn2 expressed in E10.5, decreased significantly in E16.5 but regained in E18.5, and expressed persistently in the mature mice. The results of immunoprecipitation and Western blotting technique coincided with the mRNA levels in these periods. Unfortunately, we did not find a significant protein expression in between the each stage of embryonic development, and neither in immunohistochemistry analysis. The data revealed that Lcn2 gene expressed in low level during the mouse brain development.
Subjects
Lcn2
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