Nanoplasmonic structure of a polycarbonate substrate integrated with parallel microchannels for label-free multiplex detection
Journal
Polymers
Journal Volume
13
Journal Issue
19
Date Issued
2021
Author(s)
Abstract
In this study, a multiplex detection system was proposed by integrating a localized surface plasmon resonance (LSPR) sensing array and parallel microfluidic channels. The LSPR sensing array was fabricated by nanoimprinting and gold sputter on a polycarbonate (PC) substrate. The polydimethylsiloxane (PDMS) microfluidic channels and PC LSPR sensing array were bound together through (3-aminopropyl)triethoxysilane (APTES) surface treatment and oxygen plasma treatment. The resonant spectrum of the LSPR sensing device was obtained by broadband white-light illumi-nation and polarized wavelength measurements with a spectrometer. The sensitivity of the LSPR sensing device was measured using various ratios of glycerol to water solutions with different refractive indices. Multiplex detection was demonstrated using human immunoglobulin G (IgG), IgA, and IgM. The anti-IgG, anti-IgA, and anti-IgM were separately modified in each sensing region. Various concentrations of human IgG, IgA, and IgM were prepared to prove the concept that the parallel sensing device can be used to detect different targets. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Biosensor
Multiplex detection
Nanoimprinting
Surface plasmon resonance (SPR)
Microchannels
Microfluidics
Plasma applications
Polycarbonates
Polydimethylsiloxane
Refractive index
Silicones
Surface plasmon resonance
Surface treatment
Immunoglobulin G
Localized surface plasmon resonance
Microfluidics channels
Multiplex detections
Nano-imprinting
Polycarbonate substrates
Sensing devices
Surface plasmon resonance sensing
Surface-plasmon resonance
Fluidic devices
Type
journal article