Evaluation of M2-based avian influenza virus vaccine using an adenovirus vector
Date Issued
2012
Date
2012
Author(s)
Lin, Shu-Ping
Abstract
Current vaccines to avian influenza virus can’t induce broad cross-protection against different subtypes. Therefore, the purpose of this research was to develop a vaccine which can confer cross-protection by using replication-deficient adenovirus as a vector to construct a recombinant adenovirus carrying highly conserved M2 gene (Ad/M2). The immunofluorescence assay revealed positive signal in 293A and Vero cells transduced with Ad/M2. The Ad/M2 was intranasally and intramuscularly immunized in chickens twice at a 4-week interval. Anti-M2 specific antibody was significantly higher after two weeks post primary vaccination. Challenging with AIV after two weeks post boost vaccination, the consequence of real time RT-PCR revealed AIV content was no difference between Ad/M2 group and control group in oropharyngeal swab on day 4 and day 7 post challenge. The mortality of Ad/M2 group and control group was 40% and 33.3% respectively. The conclusion is giving chickens Ad/M2 couldn’t confer protection against AIV infection.
Subjects
avian influenza virus
replication deficient adenovirus
SDGs
Type
thesis
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