Arabidopsis Micro-grafting to Study the Systemic Signaling of Nutrient Status
Part Of
Methods in Molecular Biology
Journal Volume
2665
Journal Volume
2665
Start Page
113
End Page
120
ISSN
10643745
ISBN
978-1-0716-3183-6
Date Issued
2023
Author(s)
Hsieh, En-Jung
Abstract
Grafting enables the study of systemic signals that plants use to maintain their homeostasis at the level of the whole organism. Several protocols of Arabidopsis grafting have been published over the years. These methods are limited because they either affect the overall behavior of the plant, or their throughput is low. The method presented here is based on grafting 3- to 4-days-old seedlings directly on an agar plate, without the use of hormone or collar, and can produce consistently over a hundred grafted plants per day and operator.
Subjects
Long-distance transport
Phloem
Root-to-shoot
Shoot-to-root
Systemic signaling
Whole-plant homeostasis
Xylem
Publisher
Humana Press Inc.
Type
book part