Liver Protective Effect and Mechanism of Ethanol Extract of Daylily Flower against Alcoholic Liver Damage in Mice by Enhancing Activity of Antioxidative Enzymes and Reducing Inflammation Responses
Date Issued
2014
Date
2014
Author(s)
Chen, Ying-Chu
Abstract
Liver disease is one of the most widely contracted diseases in Taiwan. There are many reasons result in liver disease, one of those are excessive alcohol consumption which will lead to alcoholic liver disease (ALD), such as in fatty liver, alcoholic hepatitis and end to cirrhosis, which results to hepatocyte dysfunction. The pathogenesis of ALD is related to lipid accumlation, oxidative stress, and inflammation. The flower of daylily (Hemerocallis fulva L.) is widely used in traditional medication and food material in eastern Asia. Previous studies indicated that the flowers of daylily are rich in variety of antioxidants such as carotenoids, flavonoids, anthocyanin, and so on. Furthermore, the extracts of daylily flowers have been reported to have the inhibition efficiency on the nitric oxide (NO) production, reducing the inducible nitric oxide synthase (iNOS) induction in lipopolysaccharide (LPS)-activated macrophages; and reative oxygen species (ROS) scavenging activity. Recent study found that the 95% ethanol extracts of daylily flowers content high level of polyphenols and exhibited better antioxidant activities than water extracts. The hypothesis of this study is ethanol extracts of daylily flower (DFE) may have the hepatoprotective effects on alcoholic liver disease mice by ameliorating the hepatic oxidative stress and inflammatory responses.. The aim of this study is to investigate the liver protective capability of ethanol extract of daylily flowers in male C57BL/6 mice which feed with Lieber-DeCarli alcohol-containing liquid diet, and its hepatoprotective mechanism. Mice were divided five groups: fed with normal liquid diet (normal control group), ethanol-containing liquid diet is (negative control group, ALD) or ethanol-containing liquid diet treated with low dosage of ethanol extract of daylily flower (DFE) at 400 mg/kg bw/day (ALD + LDFE group), high dosage of DFE at 800 mg/kg bw/day (ALD + HDFE group), and rutin which content as high dosage of DFE at 2.6 mg/kg bw/day (ALD + Rutin group) for 4 weeks. Our results showed that in comparison with the control group, the ALD group showed liver injury as evidenced by histological changes and elevation in serum biochemical, liver inflammation, and oxidative stress. These pathophysiological changes were attenuated by DFE supplementation. Further studies demonstrated an inhibitory effect of DFE on the critical role in ALD, cytochrome P450 2E1 (CYP2E1). DFE also attenuated alcohol-induced reduction in heme oxygenase-1 (HO-1) and NF-E2-related factor 2 (Nrf2). According to our results, the hepatoprotective effects of DFE were shown to be associated with antioxidative and anti-inflammatory effects as well as the downregulation expression of CYP2E1, and enhancement of HO-1 and Nrf2 expression in liver.
Subjects
酒精性肝病
金針花
氧化壓力
抗氧化
NF-E2-related factor 2
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-103-R01641032-1.pdf
Size
23.54 KB
Format
Adobe PDF
Checksum
(MD5):7575aea2ff4a5b141d18bd3a03deac35