Imbroritus primus gen. nov., sp. nov., a facultatively autotrophic bacterium from environmental water samples
Journal
International Journal of Systematic and Evolutionary Microbiology
Journal Volume
75
Journal Issue
5
ISSN
1466-5026
1466-5034
Date Issued
2025-05-13
Author(s)
Peeters, Charlotte
Steyaert, Stephanie
Wieme, Anneleen D.
Depoorter, Eliza
De Canck, Evelien
Houf, Kurt
Vandamme, Peter
Abstract
A Gram-stain-negative coccobacillus, LMG 32992T, was isolated from water that had collected in a tyre in Pingtung, Donggang Township, Taiwan. Upon preliminary 16S rRNA gene sequence analysis, it was most closely related to members of the genus Ralstonia (16S rRNA gene sequence similarities of 96.7–97.5%). The present study aimed to elucidate its taxonomic position and to propose a formal classification. To this end, the complete genome sequence was determined, and taxonomic, phylogenomic, metabolic and physiological analyses were performed. Comparative genomic analyses demonstrated that strain LMG 32992T and another unclassified strain, Burkholderiaceae bacterium PBA, which was isolated earlier from textile wastewater in Malay-sia, represented a single novel species within a novel genus of the family Burkholderiaceae. The G+C content of the LMG 32992T genomic DNA was 63.77 mol%. Genomic analyses and growth tests demonstrated that LMG 32992T had an asaccharolytic metabolism but that it was well-equipped to synthetize, if necessary autotrophically, and transform all required carbohydrates and that it used the Krebs and related cycles to generate reductive power for a heterotrophic energy metabolism. We propose the name Imbroritus primus gen. nov., sp. nov. with strain LMG 32992T (=CIP 112179T=BCRC 81361T=A30B1T) as the type strain, for this novel taxon.
Subjects
biodegradation
comparative genomics
Cupriavidus
Imbroritus primus
phylogenomics
Ralstonia
Publisher
Microbiology Society
Type
journal article