Genome-Wide Association Study in Asians Identifies Novel Loci for High Myopia and Highlights a Nervous System Role in Its Pathogenesis
Journal
Ophthalmology
Journal Volume
127
Journal Issue
12
Pages
1612-1624
Date Issued
2020
Author(s)
Meguro A.
Yamane T.
Takeuchi M.
Miyake M.
Fan Q.
Zhao W.
Mizuki Y.
Yamada N.
Nomura N.
Tsujikawa A.
Matsuda F.
Hosoda Y.
Saw S.-M.
Cheng C.-Y.
Yoshida M.
Iijima Y.
Teshigawara T.
Okada E.
Ota M.
Inoko H.
Mizuki N.
Abstract
Purpose: To identify novel susceptibility loci for high myopia. Design: Genome-wide association study (GWAS) followed by replication and meta-analysis. Participants: A total of 14 096 samples from East and Southeast Asian populations (2549 patients with high myopia and 11 547 healthy controls). Methods: We performed a GWAS in 3269 Japanese individuals (1668 with high myopia and 1601 control participants), followed by replication analysis in a total of 10 827 additional samples (881 with high myopia and 9946 control participants) from Japan, Singapore, and Taiwan. To confirm the biological role of the identified loci in the pathogenesis of high myopia, we performed functional annotation and Gene Ontology (GO) analyses. Main Outcome Measures: We evaluated the association of single nucleotide polymorphisms with high myopia and GO terms enriched among genes identified in the current study. Results: We identified 9 loci with genome-wide significance (P < 5.0 × 10–8). Three loci were previously reported myopia-related loci (ZC3H11B on 1q41, GJD2 on 15q14, and RASGRF1 on 15q25.1), and the other 6 were novel (HIVEP3 on 1p34.2, NFASC/CNTN2 on 1q32.1, CNTN4/CNTN6 on 3p26.3, FRMD4B on 3p14.1, LINC02418 on 12q24.33, and AKAP13 on 15q25.3). The GO analysis revealed a significant role of the nervous system related to synaptic signaling, neuronal development, and Ras/Rho signaling in the pathogenesis of high myopia. Conclusions: The current study identified 6 novel loci associated with high myopia and demonstrated an important role of the nervous system in the disease pathogenesis. Our findings give new insight into the genetic factors underlying myopia, including high myopia, by connecting previous findings and allowing for a clarified interpretation of the cause and pathophysiologic features of myopia at the molecular level. ? 2020 American Academy of Ophthalmology
SDGs
Other Subjects
cyclic AMP dependent protein kinase anchoring protein; genomic DNA; guanine nucleotide exchange factor; Ras protein; Rho factor; adult; allele; Article; clinical assessment; cohort analysis; controlled study; female; gene frequency; gene mapping; gene ontology; genetic association; genetic risk; genome-wide association study; genotype; high myopia; human; human tissue; Japan; Japanese (people); major clinical study; male; meta analysis (topic); nervous system; neurologic disease; odds ratio; pathogenesis; priority journal; signal transduction; Singapore; single nucleotide polymorphism; Southeast Asian; synapse; Taiwan; Asian continental ancestry group; degenerative myopia; gene locus; genetic predisposition; genetics; genome-wide association study; genotyping technique; middle aged; neurologic disease; single nucleotide polymorphism; Asian Continental Ancestry Group; Female; Genetic Loci; Genetic Predisposition to Disease; Genome-Wide Association Study; Genotyping Techniques; Humans; Japan; Male; Middle Aged; Myopia, Degenerative; Nervous System Diseases; Polymorphism, Single Nucleotide; Singapore; Taiwan
Publisher
Elsevier Inc.
Type
journal article