Simultaneous three-dimensional imaging of multi-focal microscopy
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
10251
ISBN
978-1510610033
Date Issued
2017
Author(s)
Abstract
High-speed microscopy three-dimensional (3D) microscopy based on trans-illumination is implemented with an amplitude light modulator placed at the Fourier plane of the system. The phase of an incident wave-front is modified and encoded with a defocus parameter to divert the light onto different portion of an image plane depending on their diffraction order and depth positions. The design of the grating pattern for the light modulated is discussed through the simulation and the experiment. 3D imaging capability is demonstrated through the experiment.
SDGs
Publisher
SPIE
Type
conference paper