Genetic variants of IL-6 and its receptor are not associated with schizophrenia in Taiwan
Journal
Neuroscience Letters
Journal Volume
468
Journal Volume
468
Journal Issue
3
Journal Issue
3
Pages
330-333
Start Page
330
End Page
333
ISSN
0304-3940
Date Issued
2010-01-14
Author(s)
Liu Y.-L.
Fann C.S.-J.
Yang W.C.
Chen Y.-H.
Tseng L.-J.
Liu S.-K.
Chan H.-Y.
Chen J.-J.
Abstract
The pathophysiological process of schizophrenia is still unclear. The levels of interleukine-6 (IL-6) and its receptor, soluble IL-6R, have been reported to be elevated in the plasma and cerebrospinal fluid of schizophrenic patients. In this study, we tested the association of genetic variants of IL-6 and IL-6R with schizophrenia. Genotyping of three single nucleotide polymorphisms (SNP) for each IL-6 (IL-6-1, IL-6-2, and IL-6-3) and IL-6R (rs4845617 = IL-6R1, rs4553185 = IL-6R2, and rs4379670 = IL-6R3) gene was performed in 100 patients with schizophrenia and 113 normal controls. The polymorphisms of IL-6R2 were genotyped using Tetra-primer ARMS PCR. IL-6R3 polymorphisms were genotyped using restriction fragment length polymorphism (RFLP) with Apo I enzyme as the restriction enzyme. All other polymorphisms were genotyped using the direct sequencing method. We found a di-nucleotide haplotype block and a tri-nucleotide haplotype block in the genes of IL-6 and IL-6R, respectively. All six SNPs and their haplotypes failed to show a significant association with schizophrenia. The IL-6-2 SNP showed a nominally significant association with the positive symptoms of schizophrenia (p = 0.0472). We conclude that the genetic variants of IL-6 and IL-6R are not associated with schizophrenia. In order to verify this result, further study using a larger sample size and exploring the association between the genotype of IL-6-2 and plasma level of IL-6 is recommended. ? 2009 Elsevier Ireland Ltd. All rights reserved.
SDGs
Other Subjects
dinucleotide; interleukin 6; interleukin 6 receptor; trinucleotide; 3' untranslated region; adult; article; controlled study; disease predisposition; disease severity; enzyme regulation; exon; female; gene frequency; gene locus; genetic association; genetic marker; genetic variability; genotype; human; intron; major clinical study; male; negative syndrome; phenotype; positive syndrome; priority journal; promoter region; restriction fragment length polymorphism; schizophrenia; single nucleotide polymorphism; Taiwan; Adult; Asian Continental Ancestry Group; Female; Genetic Association Studies; Genetic Predisposition to Disease; Humans; Interleukin-6; Male; Polymorphism, Single Nucleotide; Receptors, Interleukin-6; Schizophrenia; Taiwan
Type
journal article