Immunological activity of extracts from the marine brown alga, Endarachne binghamiae
Date Issued
2005
Date
2005
Author(s)
Lee, Hui-Ting
DOI
zh-TW
Abstract
Various extracts of the marine brown alga Endarachne binghamiae were assayed in vitro for immunological activity against murine macrophages and human T cells. In addition, the immune-active substances were extracted, purified and characterized biochemically. It appeared that all extracts used, including saline, hot-water and aqueous ethanolic extracts, could significantly stimulate macrophage and T cell proliferation to various extents. Sodium alginate had greater activity than alginic acid and isopropanol-precipitated polysaccharide, with maximal activity, i.e. proliferation increased by 70~100 %, at 0.05~0.1 µgmL-1. A novel protein assigned as EBP ( Endarachne binghamiae protein) was obtained after being extracted in phosphate buffered saline, precipitated by saturated ammonium sulfate and finally purified by gel filtration and ion-exchange column chromatography. The purified protein had molecular weight of 69 kDa. It was a monomer glycoprotein, containing 20.37 % carbohydrate moiety. EBP also stimulated strongly phagocytes and lymphocytes to proliferate, with maximal proliferation, i.e. by twofold, at 0.04~0.25 µgmL-1. Further assays showed distinct increases of cytokines, i.e. TNF–α, IFN–γ, and nitric oxide, generated and released from macrophages and T cells in the cultures with EBP, using sandwich ELISA method. EBP was rather stable in immunity while subjected to 121 ℃ and a wide range of pH values (2.0~13.0). The above findings suggested that the reported alga is a promising source of proteins and polysaccharides used for immunostimulation and immuno-modulation in therapy and medicine.
Subjects
小海帶
Endarachne binghamiae
SDGs
Type
thesis
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