Genetic and Functional Study of the Genes for the Capsular Polysaccharide Biosynthesis of Klebsiella pneumoniae
Date Issued
2011
Date
2011
Author(s)
Ho, Jin-Yuan
Abstract
The growing number of Klebsiella pneumoniae infections, commonly acquired in hospitals, has drawn great concerns. It has been shown that the K1 and K2 capsular serotypes are the most detrimental strains, particularly to those with diabetes. The K1 cps (capsular polysaccharide) locus in the NTUH-K2044 strain of the pyogenic liver abscess (PLA) K. pneumoniae has been identified recently, but little is known about the functions of the genes therein. The active components of primary pyogenic liver abscess (PLA) Klebsiella pneumoniae in stimulating cytokine expressions in macrophages are still unclear from the host immunity and bacterial virulence, although statistical studies in pathogenesis showed that magA, rmpA, and liver abscess with diabetes, bacteremia and metastatic complications are related. In this dissertation, we demonstrated the functions of nine annotated genes in the UDP-galacturonate and GDP-fucose biosynthetic pathways. We pursued the relationship between the genes in the CPS gene cluster and virulence by studying the trisaccharide biosynthesis and decoration. In addition to the biochemical approach, we also paid attention to these genes in genetic level. Gene expression patterns of three gene deletion (kp3706, kp3709, and kp3712) strains showed a novel broad–range of gene silencing, ranging from kp3705 to kp3767. Metabolite analysis and antibiotic resistance supported the silencing effect. In summary, this work characterized the functions of genes in NDP-sugar biosynthesis and revealed an unprecedented broad spectra gene silencing effect.
Subjects
Klebsiella pneumoniae
cps biosynthesis
SDGs
Type
thesis
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