The Development of Hydrodynamic Flow Assisted Double Junction Interface for Signal Improvement in CE-MS using Negatively Charged Nonvolatile Additives
Date Issued
2015
Date
2015
Author(s)
Huang, Ya-Ju
Abstract
Phenols widely used in plastics industry but they were major pollutants when entering the environment. Sodium dodecyl sulfate (SDS) was required for the analysis of phenols by micellar electrokinetic chromatography (MEKC); however, SDS would cause crystals to block the spray tip and also suppress the analyte signals during ionization process in mass interface. In this study, the replacement of SDS by ADS (ammonium dodecyl sulfate) could avoid crystals on the spray tip, and a hydrodynamic flow assisted double junction interface was utilized to alleviate ion suppression from micelles in MEKC-ESI-MS. The transfer column coated with polyvinyl alcohol (PVA) in the interface could decrease the electroosmotic flow (EOF) significantly; therefore, the EOF could be ignored. By introducing organic solvent, the micelles would be collapsed, the ADS would be retained by applying electric field of transfer column, and the analytes would keep moving forward into ESI-MS interface via transfer column by hydrodynamic flow. Consequently, the problem of signal suppression by ADS was alleviated successfully.
Subjects
MEKC-ESI-MS
hydrodynamic flow assisted double junction interface
ion suppression
phenols
ammonium dodecyl sulfate (ADS)
Type
thesis
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