Antioxidative Activity and Active Components of Longan (Dimocarpus longan Lour.) Flower Extracts
Resource
Journal of Agricultural and Food Chemistry, 56(16), 7010-7016
Journal
Journal of Agricultural and Food Chemistry
Pages
7010-7016
Date Issued
2008
Date
2008
Author(s)
Hsieh, Meng-Chieh
Shen, Yi-Jane
Kuo, Yueh-Hsiung
Hwang, Lucy Sun
Abstract
Three different solvent extracts (methanol, ethyl acetate, and n-hexane) of longan ( Dimocarpus longan Lour.) flowers were assayed with three different antioxidant capacity methods, namely, the DPPH free radical scavenging effect, the oxygen radical absorbance capacity (ORAC) assay, and the inhibition of Cu(2+)-induced oxidation of human low-density lipoprotein (LDL). It was revealed that the methanol extract has the best antioxidative activity, followed by ethyl acetate and n-hexane extracts. The methanol extract was separated by liquid-liquid partition into n-hexane, ethyl acetate, n-butanol, and water fractions. The ethyl acetate fraction was found to have the highest activity of delaying LDL oxidation. After silica gel column chromatography, the fraction having a superior activity was identified as containing two major compounds, (-)-epicatechin and proanthocyanidin A2.
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Type
journal article
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