A miniaturized germanium-doped silicon dioxide-based surface plasmon resonance waveguide sensor for immunoassay detection
Journal
Biosensors and Bioelectronics
Journal Volume
22
Journal Issue
4 SPEC. ISS.
Pages
519-525
Date Issued
2006
Date
2006
Author(s)
Huang, Jhen-Gang
Lee, Chen-Lung
Chuang, Tsung-Liang
Wang, Way-Seen
Juang, Rong-Huey
Wang, Ching-Ho
Abstract
A surface plasmon resonance (SPR) waveguide immunosensor fabricated by germanium-doped silicon dioxide was investigated in this study. The designed waveguide sensor consisted of a 10 μm SiO2 substrate layer (n = 1.469), a 10 μm Ge-SiO2 channel guide (n = 1.492) and a 50 nm gold film layer for immobilization of biomolecules and SPR signal detection. The resultant spectral signal was measured by a portable spectrophotometer, where the sensor was aligned by a custom-designed micro-positioner. The results of the glycerol calibration standards showed that the resonance wavelength shifted from 628 to 758 nm due to changes of refractive index from 1.36 to 1.418. Flow-through immunoassay on waveguide sensors also showed the interactions of protein A, monoclonal antibody (mAb ALV-J) and avian leucosis virus (ALVs) resulted in wavelength shifting of 4.17, 3.03 and 2.18 nm, respectively. The SPR dynamic interaction could also be demonstrated successfully in 4 min as the sensor was integrated with a lateral flow nitrocellulose strip. These results suggest that SPR detection could be carried out on designed waveguide sensor, and the integration of nitrocellulose strip for sample filtering and fluid carrier would facilitate applications in point-of-care portable system. © 2006 Elsevier B.V. All rights reserved.
Subjects
Immunosensor; Surface plasmon resonance; Waveguide
Other Subjects
Doping (additives); Germanium; Silica; Spectrophotometers; Surface plasmon resonance; Waveguides; Avian leucosis virus (ALV); Fluid carriers; Point-of-care portable systems; Waveguide sensors; Immunosensors; germanium; silicon dioxide; analytic method; article; calibration; coated particle; electroimmunoassay; immobilization; material coating; optical biosensor; point of care testing; refraction index; signal detection; surface plasmon resonance; Avian leukosis virus
Type
journal article
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