Endothelial progenitor cells derived from wharton's jelly of human umbilical cord attenuate ischemic acute kidney injury by increasing vascularization and decreasing apoptosis, inflammation, and fibrosis
Journal
Cell Transplantation
Journal Volume
24
Journal Issue
7
Pages
1363-1377
Date Issued
2015
Author(s)
Liang C.-J.
Shen W.-C.
Chang F.-B.
Young G.-H.
Tseng Y.-C.
Peng Y.-S.
Abstract
Ischemia-reperfusion (I/R) injury to the kidney, a major cause of acute renal failure in humans, is associated with a high mortality, and the development of a new therapeutic strategy is therefore highly desirable. In this study, we examined the therapeutic potential of implantation of endothelial progenitor cells (EPCs) isolated from Wharton's jelly of human umbilical cords in the treatment of renal I/R injury in mice. To visualize the localization of the transplanted EPCs, the cells were labeled with Q-tracker before injection into the renal capsule. Mice with renal I/R injury showed a significant increase in blood urea nitrogen and creatinine levels, and these effects were decreased by EPC transplantation. The kidney injury score in the mice with I/R injury was also significantly decreased by EPC transplantation. EPC transplantation increased the microvascular density, and some of the EPCs surrounded and were incorporated into microvessels. In addition, EPC transplantation inhibited the I/R-induced cell apoptosis of endothelial, glomerular, and renal tubular cells, as demonstrated by TUNEL staining, and significantly reduced reactive oxygen species production and the expression of the inflammatory chemokines macrophage inflammatory protein-2 and keratinocyte-derived cytokine, as shown by immunostain-ing and ELISA. Moreover, EPC transplantation reduced I/R-induced fibrosis, as demonstrated by immuno-staining for S100A4, a fibroblast marker, and by Jones silver staining. To our knowledge, this is the first report that transplantation of EPCs from Wharton's jelly of human umbilical cords might provide a novel therapy for ischemic acute kidney injury by promoting angiogenesis and inhibiting apoptosis, inflammation, and fibrosis. ? 2015 Cognizant Comm. Corp.
SDGs
Other Subjects
calvasculin; chemokine; creatinine; cytokine; macrophage inflammatory protein 2; nitrogen; reactive oxygen metabolite; urea; acute kidney failure; animal experiment; animal model; animal tissue; apoptosis; Article; attenuation; blood sampling; cell culture; cell density; cell isolation; controlled study; creatinine blood level; endothelial progenitor cell; endothelial progenitor cell transplantation; enzyme linked immunosorbent assay; glomerulus; human; human cell; immunohistochemistry; in vitro study; in vivo study; inflammation; injection; Jones silver staining; keratinocyte; kidney blood flow; kidney capsule; kidney fibrosis; kidney function; kidney injury score; kidney ischemia; kidney parenchyma; kidney tubule cell; male; microvasculature; mouse; nonhuman; priority journal; protein expression; staining; stem cell transplantation; TUNEL assay; umbilical cord; urea nitrogen blood level; Wharton jelly; Acute Kidney Injury; animal; apoptosis; endothelial progenitor cell; fibrosis; inflammation; metabolism; reperfusion injury; umbilical cord; Wharton jelly; Mus; Mus; Acute Kidney Injury; Animals; Apoptosis; Endothelial Progenitor Cells; Fibrosis; Humans; Inflammation; Mice; Reperfusion Injury; Umbilical Cord; Wharton Jelly
Publisher
Cognizant Communication Corporation
Type
journal article