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SUMO Modifications Control Assembly of Synaptonemal Complex in Meiosis of Saccharomyces cerevisiae
Date Issued
2006
Date
2006
Author(s)
Cheng, Chun-Hsu
DOI
zh-TW
Abstract
Synaptonemal complex (SC) is a proteinaceous complex that apparently mediates synapsis between homologous chromosomes during meiotic prophase. In S. cerevisiae, the Zip1 protein is the integral component of SC. In the absence of a DNA double-strand break or the SC initiation protein Zip3, Zip1 proteins aggregate to form a polycomplex (PC). In addition, Zip1 is also responsible for DSB-independent non-homologous centromere coupling at early meiotic prophase. We report here that Zip3 is a SUMO E3 ligase and that Zip1 is a binding protein for SUMO-conjugated products. Our results also suggest that at early meiotic prophase, Zip1 interacts with Zip3-independent Smt3 conjugates (e.g., Top2) to promote non-homologous centromere coupling. At and after mid-prophase, the Zip1 protein begins to associate with Zip3-dependent Smt3 conjugates (e.g., Red1) along meiotic chromosomes in the wild-type cell to form SCs and with Smt3 polymeric chains in the zip3 mutant to form PCs.
Subjects
減數分裂
類泛素化
meiosis
synaptomenal complex
sumoylation
Type
other
File(s)
No Thumbnail Available
Name
ntu-95-R93b46011-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):96f6e9eb6ad7ce4c6d1f42a129688073