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嚴重急性呼吸道症候群的免疫致病機轉(第二年)─(子計畫四)SARS病人抗上皮細胞及內皮細胞自體抗體致病角色之探討
Date Issued
2005
Date
2005
Author(s)
楊曜旭
DOI
932751B002006Y
Abstract
The severe acute respiratory syndrome (SARS) is caused by infection with the
SARS-associated coronavirus (SARS-CoV) and characterized by severe pulmonary
inflammation and fibrosis. In this study, the development of autoantibodies against
human epithelial cells and endothelial cells in patients with SARS at different time
periods (the first week: phase I, one month after the disease onset: phase II/phase III)
were investigated. Antibodies in sera of patients and healthy controls against 1) A549
human pulmonary epithelial cell-line, 2) human umbilical venous endothelial cells
(HUVEC), 3) primary human pulmonary endothelial cells (HPEC) were detected by
cell-based ELISA and indirect immunofluorescence staining. The results revealed that
serum levels of IgG anti-A549 cells antibodies, IgG anti-HUVEC antibodies, and IgM
anti-HPEC antibodies were significantly higher in SARS patients at phase II/phase III
than those in healthy controls. Sera from SARS patients at phase II/phase III could
mediate complement dependent cytotoxicity against some A549 cells and HPEC. It is
concluded that some autoantibodies against human epithelial cells and endothelial
cells would be developed after SARS-CoV infection and this phenomenon may
indicate post-infectious cellular injury and also induce SARS-induced
immunopathology.
SARS-associated coronavirus (SARS-CoV) and characterized by severe pulmonary
inflammation and fibrosis. In this study, the development of autoantibodies against
human epithelial cells and endothelial cells in patients with SARS at different time
periods (the first week: phase I, one month after the disease onset: phase II/phase III)
were investigated. Antibodies in sera of patients and healthy controls against 1) A549
human pulmonary epithelial cell-line, 2) human umbilical venous endothelial cells
(HUVEC), 3) primary human pulmonary endothelial cells (HPEC) were detected by
cell-based ELISA and indirect immunofluorescence staining. The results revealed that
serum levels of IgG anti-A549 cells antibodies, IgG anti-HUVEC antibodies, and IgM
anti-HPEC antibodies were significantly higher in SARS patients at phase II/phase III
than those in healthy controls. Sera from SARS patients at phase II/phase III could
mediate complement dependent cytotoxicity against some A549 cells and HPEC. It is
concluded that some autoantibodies against human epithelial cells and endothelial
cells would be developed after SARS-CoV infection and this phenomenon may
indicate post-infectious cellular injury and also induce SARS-induced
immunopathology.
Subjects
SARS
autoantibodies
epithelial cell
endothelial cell
cytotoxicity
SDGs
Publisher
臺北市:國立臺灣大學醫學院小兒科
Coverage
計畫年度:93;起迄日期:2004-07-01/2005-06-30
Type
report
File(s)
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932751B002006Y.pdf
Size
127.37 KB
Format
Adobe PDF
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