Investigation of Temperature Effect on Fluorescent Imaging Using Fluorescent Microcapsules and Cryomicroscope
Date Issued
2011
Date
2011
Author(s)
Chen, Yu-Chou
Abstract
Measuring temperature is a very important issue. Recently, using optical technologies to measure temperature has been developed, such as fluorescent imaging. This method can overcome the problem that objects under the situation of high voltage or high magnetic field, which normal thermometer can’t apply. In this research we using fluorescent imaging technology to measure temperature. The main idea is using thermoelectric cooler cryomicroscope which can control temperature easily to simulates temperature we needed. The samples in this research are fluorescent microcapsules, then excited it by blue LED and observed intensity of fluorescent image in different temperature. This research can be divided into two parts. The first part is to investigate the effect of different conditions to fluorescent microcapsules, such as size of microcapsules, distance of excited light, concentration of fluorescent solution. The second part is to establish measurement models by experimental results, then using the models to measure the temperature. The measurable range is -15 ~ 90 ℃. The models in this research are based on method of fluorescent decay, method of single fluorescent intensity, and method of fluorescent ratio. Each method has best RMSE = 9.74 ℃, 5.04 ℃, and 3.41 ℃. And R2 = 0.890, 0.979, and 0.972. The results show fluorescent imaging technology can really apply to temperature measurement, also the best model is method of fluorescent ratio, and it more repeatable than other methods.
Subjects
Fluorescence Temperature Measurement
Fluorescent Microcapsules
Thermoelectric Cooler Cryomicroscope
Type
thesis
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